G5882

Sigma-Aldrich

戊二醛 溶液

Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative

别名：1,5-戊二醛, 五碳双缩醛 溶液

线性分子式：OHC(CH2)3CHO

CAS号：111-30-8

分子量：100.12

Beilstein：605390

MDL编号：MFCD00007025

PubChem化学物质编号：24895235

NACRES：NA.21

属性

质量水平

300

类型

Grade I

形式

liquid

浓度

25% in H2O

technique(s)

electron microscopy: suitable (fixative)

颜色

colorless

useful pH range

2.9

mp

-10 °C ((14 °F ))

溶解性

water: soluble at 20 °C

运输

dry ice

储存温度

−20°C

SMILES string

[H]C(CCCC([H])=O)=O

InChI

1S/C5H8O2/c6-4-2-1-3-5-7/h4-5H,1-3H2

InChI key

SXRSQZLOMIGNAQ-UHFFFAOYSA-N

说明

一般描述

Glutaraldehyde (Glutaric dialdehyde, Pentane-1,5-dial) is an aliphatic dialdehyde widely utilized as a versatile 

biochemical reagent. Glutaraldehyde solutions contain both monomers and polymers with the difference in their 

fixation properties. Its applications include serving as a protein crosslinker, facilitating enzyme immobilization in 

microscopy, histochemistry, and cytochemistry. This compound acts as an amine-reactive homobifunctional 

crosslinker and a cell fixative before SDS-PAGE, staining, or electron microscopy, making it suitable for diverse 

morphological studies.

Glutaraldehyde efficiently crosslinks amine and hydrazine derivatives to proteins and other amine-containing 

polymers. Notably, it has been used in certain reactions to directly couple biotin hydrazides to nucleic acids, 

suggesting potential applications in conjugating fluorescent hydrazides and hydroxylamines to DNA. 

In histology studies, Glutaraldehyde is employed to preserve tissue sections, preparing them for closer examination.

应用

戊二醛溶液已用于：

固定血液样品，进行扫描电镜(SEM)观察

固定支架进行SEM分析

在药物实验中处理K562（白血病）细胞，永久交联细胞组分

生化/生理作用

戊二醛在生物医学研究中可用来固定细胞。还可作为健康和病理红细胞的研究工具。戊二醛广泛用于消毒各种医疗器械，

包括内窥镜、透析管、血液透析比例混合和透析液供给系统、腔内探头、换能器、麻醉、呼吸治疗设备以及一次性腹腔镜

塑料套管的再利用。

特点和优势

Versatile and adaptable for wide variety of research applications

Ready-made solution reduces the need for preparation time

其他说明

推荐用于需要高纯度的技术。